Read e-book online Applied Biochemistry and Bioengineering. Enzyme Technology PDF

By Lemuel Wingard (Eds.)

ISBN-10: 0120411024

ISBN-13: 9780120411023

ISBN-10: 0323156010

ISBN-13: 9780323156011

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Additional resources for Applied Biochemistry and Bioengineering. Enzyme Technology

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2. T h e first step usually is to concentrate the enzyme by evaporation or ultrafiltration. Evaporation is performed in traditional or custom-built multistage vacuum evaporators. T h e liquid must b e kept at a low tem­ perature ( 3 0 ° - 5 0 ° C ) . Nevertheless it is difficult to prevent activity losses o f — 1 0 % even for very stable enzymes. An additional disadvan­ tage o f this method is that the low temperature makes microbial growth possible, so that frequent cleaning o f equipment and careful control o f the product are necessary.

T h e loss o f rennet activity is insignificant (Schleich, 1971). Another example is the removal o f transglucosidase activity from glucoamylase preparations. T h i s has b e e n a serious problem in indus­ try, and over the years many patent applications have b e e n filed on methods to solve the problem. A n u m b e r o f methods are listed in Table V I I I . S. Patent No. 5-3 Treatment with chloroform Treatment with hetero poly(acid)s such as phosphomolybdic acid 46 Knud Aunstrup 3. Finishing of Solid Enzyme Preparations T h e dried preparations from salt or solvent precipitation form large lumps which are unattractive to sell.

A. (1976). In "Advances in Microbial Physiology" (A. H. Rose and D. W. ), Vol. 14, pp. 1-92. Academic Press, New York. Dean, A. C. R. (1972). J. Appl Chem. Biotechnol 22, 245. Delente, J . , Johnson, J . , Kuo, M. J . , O'Connor, R. J . , and Weeks, L. E . (1974). Biotechnol. Bioeng. 16, 1227. Demain, A. L. (1972). Biotechnol. Bioeng. Symp. 3, 21. Diers, I. (1976). In "Continuous Culture 6: Applications and Fields" (A. C. R. Dean, C. G. T. Evans, and J. ), pp. 208-225. Ellis Horwood, Chichester.

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Applied Biochemistry and Bioengineering. Enzyme Technology by Lemuel Wingard (Eds.)


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